LeukoTrace
Our Innovation
We discovered that four types of leukemia, CML, ALL, AML, and CLL, share the biomarker BCR-ABL1 fusion protein. Using fluorescent conjugation to detect the fusion protein, we were able to create LeukoTrace, a novel screening tool for leukemia.
Philadelphia Chromosome
The ABL1 gene from chromosome 9 and BCR gene from 22 break off and swap places, creating a fusion protein with abnormal tyrosine kinase activity [35]. This activity leads to abnormal cell signaling pathways and uncontrolled cell growth, ultimately cancer.
Rapid Antibody Test
Similar to a rapid antigen test, this method involves collecting a sample, mixing it into a buffer, and dropping the solution onto a test cassette. However, it differs by detecting antibodies instead of antigens, using blood collection instead of swabbing, and displaying results under UV light [40, 41].
Fluroescent Conjugated BCR-ABL1 Antibodies
Antibodies are combined with fluorescent dye and purified to create conjugated antibodies. This process is key to our technology, as the fluorescent dye tags the fusion protein, making it visible. These conjugated antibodies are then used to visualize patient results at a cellular level [16].
Bicarbonate Buffer Solution
The bicarbonate buffer solution maintains optimal pH environment during protein labeling process, ensuring reactive proteins and enhances reaction for effectiveness of fluorescence reactions. Additionally, it stabilizes proteins, preventing denaturation [10].
How to Use
1) Wash and dry your hands thoroughly to remove any substances that may affect the reading
2) Use the lancet in the kit to gently prick the side of your fingertip
3) Draw 0.2 mL of blood from the pricked finger using the pipette
4) Mix the blood sample with the buffer solution in the dropper tube
5) Squeeze out the blood sample from the pipette into the hole on the test cassette
6) Set a timer for 10-15 minutes and wait for the results
7) Afterwards, shine the UV light on the marked area (show area B)
8a) If you see two lines illuminated, then that means the sample is positive, meaning you’re at risk for developing AML, CML, ALL, or CLL
​
8b) However, if it shows one line, then that would mean the protein marker was not found within the blood sample.
