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Breakthroughs
Step 1: To create mutations that drive overexpression of poplar RCA, first clone wild-type poplar RCA gene and its 5’ upstream region (including promoter and enhancer sequences) into the plasmid from phytopathogen, Agrobacterium (nature’s most effective “plant genetic engineer”).
Click through the 5 steps to generate the breakthroughs needed for photosynthesis-enhanced trees with modified RCA:​
- Site-Directed mutagenesis will be used to generate overexpressing wild-type RCA
- Graph Neural Networks will be used to generate overexpressing thermostable RCA
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